Familial association of thrombopathia and antihemophilic factor (AHF, factor VIII) deficiency.

Immunologic studies of antihemophilic factor (AHF, factor VIII). V. Immunologic properties of AHF subunits produced by salt dissociation.

Quantitative assay of a plasma factor deficient in von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content.

In a previous paper, we showed that the abnormality of ristocetin-induced platelet aggregation in platelet-rich plasma in 10 patients with von Willebrand's disease could be corrected by a factor in normal plasma that was present in the same fractions as factor VIII procoagulant activity (antihemophilic factor, AHF, VIII(AHF)) when prepared by chromatography on Bio-Gel 5 M (Bio-Rad Laboratories,...

Synthesis of antihemophilic factor antigen by cultured human endothelial cells.

Antihemophilic factor (AHF, Factor VIII) antigen has been demonstrated in cultured human endothelial cells by immunofluorescence studies using monospecific rabbit antibody to human AHF. Control studies with cultured human smooth muscle cells and human fibroblasts were negative. By radioimmunoassay it was demonstrated that cultured human endothelial cells contain AHF antigen which is released in...

The Relationship of the Properties of Antihemophilic Factor ( Factor VIII ) That Support Ristocetin - Induced Platelet Agglutination

A monoclonal antibody to human antihemophilic factor (AHF. factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody. harvested from culture medium or ascites fluid. reacted with purified AHF and with plasmas from normal subjects or classic hemophiliacs, as measured by enzymelinked immuonsorbant assay (ELISA). but not with plasmas from p...

Properties of the platelet retention (von Willebrand) factor and its similarity to the antihemophilic factor (AHF).